Data Analysis of the F-SHARP Multiphocal Method in Biological Tissues

Description:
The student will analyze experimental data obtained using the F-SHARP (Focus Scanning Holographic Aberration Probing) method from fluorescent neurons in the brain tissue of Danionella translucida. The goal is to process and analyze the data to extract quantitative information on the improvement of optical focusing in scattering media.

Required Knowledge:

• Understanding basic concepts of optical microscopy and laser propagation in tissues
• Programming in Matlab or Python
• Data analysis and visualization

Suggested Bibliography:

• Papadopoulos, I. N. et al. Scattering compensation by F-SHARP. Nature Photonics (2017).
• Papadopoulos, I. N. et al. Dynamic conjugate F-SHARP microscopy. Light: Science and Applications (2020).
• Denk, W. et al. Two-photon laser scanning fluorescence microscopy. Science (1990).